lickometer

[Med_Support]

Flienard

Hi, everyone,
I am tying to measure number of licks, time of licks and time between licks by IRT but it doe not work.
I’ve attached the program I use. Someone could help me please to found what is wrong?

Thank

Fabienne

lickingtest12a

[Med_Support]

Jim Frei

Hi Fabienne,

This code should do what you want or get you started at least:

\Inputs
^rightLick = 1
^leftLick  = 2


\Control variables
VAR_ALIAS Session Timer (sec)   = A(0) \Default = 600
VAR_ALIAS Bin Timer (sec)       = A(1) \Default = 60

^sessionTimer = 0
^binTimer   = 1

\Data variables
\B(E) = Right licks/bin
\C(E) = Left licks/bin
\D(F) = Time of each right lick
\G(H) = Time of each left lick
\I(J) = Array of ITR times 

DIM A = 1
DIM B = 100
DIM C = 100
DIM D = 10000
DIM G = 10000
DIM I = 10000

\Working variables
\E = Bin number
\F = Total right licks
\H = Total left licks
\J = I() index

\R = IRT timer
\S = Session timer
\T = Bin timer

\Z-Pulses
\Z1 = Start program
\Z2 = End program

\*********************************************
\     Initialize values
\*********************************************
S.S.1,
S1,
    0.001": SET A(^sessionTimer) = 600, A(^binTimer) = 60;
            SET E = 1, J = 1;
            SET B(E+1) = -987.987, C(E+1) = -987.987;
            SET D(F+1) = -987.987;
            SET G(H+1) = -987.987;
            SET I(J) = -987.987 ---> S2

S2,
    #START: Z1 ---> SX


\********************************************
\    Session timer
\********************************************
S.S.2,
S1,
    #Z1: ---> S2

S2,
    0.01": SET S = S + 0.01; SHOW 1, Session Timer, S;
           IF S >= A(^sessionTimer) [@T, @F]
                @T: Z2 ---> S1
                @F: ---> SX


\**********************************************
\   Bin timer
\**********************************************
S.S.3,
S1,
    #Z1:  ---> S2

S2,
    0.01": SET T = T + 0.01; SHOW 6, Bin Timer, T;
           IF T >= A(^binTimer) [@T, @F]
                @T: ADD E; SET T = 0;
                    SET B(E) = 0, C(E) = 0;
                    SET B(E+1) = -987.987, C(E+1) = -987.987---> S2
                @F: ---> SX


\*********************************************
\    Record licks & IRT timer
\*********************************************
S.S.4,
S1,
    #Z1: ---> S2

S2, \Record right licks
    #R^rightLick: ADD F;
                  SET D(F) = S;
                  SET D(F+1) = -987.987;
                  ADD B(E);
                  SET I(J) = R;
                  ADD J; SET I(J) = -987.987;
                  SET R = 0 ---> S2

    \Record left licks
    #R^leftLick: ADD H;
                 SET G(H) = S;
                 SET G(H+1) = -987.987;
                 ADD C(E);
                 SET I(J) = R;
                 ADD J; SET I(J) = -987.987;
                 SET R = 0 ---> S2



    0.01": SET R = R + 0.01; SHOW 8, IRT Timer, R ---> SX




\******************************************
\    Update the display
\********************************************
S.S.31,
S1,
    0.1": SHOW 2, Total Right Licks, F;
          SHOW 3, Total Left Licks, H;
          SHOW 7, Bin Number, E ---> SX





\*********************************************
\   End program
\********************************************
S.S.32,
S1,
    #Z2: ---> S2

S2,
    1": ---> STOPABORTFLUSH

[Med_Support]

Flienard

Hi, Jim
Thank you so much
I will test your program now. And sure it will help me;
Thank you again
Fabienne

 

…

Hi Jim,
We are using ENV-251L with photobeam sensor, I wonder if I should use beambreak code?

Fabienne

[Med_Support]

Jim Frei

Hi Fabienne,

I misunderstood your question, when you said time of licks I thought you meant at what point in time the lick occurs, not how long the animal’s head is in the dispenser. Give me a little bit and I’ll see if I can adjust the code.

Jim

…

Hi Fabienne,

Here’s some sample code on how to time the length of an input. If you need more help let us know.

How to time the length of an input
\***************************************************
\ RESPONSE DEFINED - 20ms BREAK, 20ms RELEASE
\***************************************************
S.S.2,
S1, \ Inputs in Level Mode generate an input "count" on each
\ interrupt. With a 10 ms system resolution 2 counts
\ will be reached in 20 ms. The Z3 pulse is used to
\ signal a completed Response. The second statement
\ resets the counter every 20 ms so that a partial
\ Response of less than 20 ms will not be counted.
#R1: ADD Y; IF Y >= 2 [@Response, @NoResponse]
@Response: SET Y = 0; Z3 ---> S2
@NoResponse: ---> SX
0.02": SET Y = 0 ---> S1
S2, \ As long as the input is broken the second statement
\ causes a re-entry to this State. This resets the
\ internal 20 ms timer so it never times out. When the
\ input is released for 20 ms the timer times out and a
\ Z4 pulse signals the release.
0.02": Z4 ---> S1
#R1: ---> S2
\***************************************************
\ RESPONSE TIMER
\***************************************************
S.S.3,
S1,
#START: SET Z(I) = -987.987 ---> S2
S2, \ Wait for a signal that the input has been broken.
#Z3: SET T = 0 ---> S3
S3, \ Wait for signal that the input has been released.
\ Record the length of the Response.
0.01": SET T = T + 0.01 ---> S3
#Z4: SET Z(I) = T; ADD I; SET Z(I) = -987.987 ---> S2

[Med_Support]

Flienard

Hi Jim,
You are right we need the time when the licks occur. But when I use the program you send us it gives me plenty of values for one lick and the array of irt is plenty of “0” and “0.01”, I do not understand why. This is why I thought the beam break code should ameliorate the result but I do not know if it is correct, if you said that the beam break code is used to measure the time spend by the animal in the dispenser.

 

Fabienne

[Med_Support]

Jim Frei

Fabienne see my second post above please.

Jim

[Med_Support]

Flienard

I do not understand why the first program you sent us does not work, do you have any idea?

 

Fabienne

…

Hi Jim,
Your first program works well now. Our Superport input settings JP3 and JP4 were placed on the level mode. I have changed for the transition mode and it works well now.

 

Fabienne

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